Research Programs

Faculty and staff from Biomanufacturing Training and Education Center (BTEC), departments within the College of Engineering, and the College of Textiles are collaborating with faculty from the Technical University of Denmark (DTU) on projects that range from cell factory engineering to upstream bioreactor design and optimization and to downstream capture and purification operations. Projects, principal investigators (PIs) and co–PIs are shown below.

Projects

Perfusion system for high yield production of complex proteins and glycoproteins

PI: Steffen Goletz, DTU Bioengineering
Co-PI: Laurie Overton, BTEC

Next-gen brewing: Therapeutic activity and manufacturability of probiotic yeast

PI: Nathan Crook, NC State CBE
Co-PIs: José Luis Martínez Ruiz, DTU Bioengineering; Christopher Workman, DTU Bioengineering

Novel biosensors for product quality attributes and contaminant detection

PI: Michael Daniele, NC State BME/ECE
Co-PIs: Stefano Menegatti, NC State CBE; Krist Gernaey, DTU Chemical Engineering

Yeast cell factory design and high-throughput screening for therapeutic protein production

PI: José Luis Martínez Ruiz, DTU Bioengineering
Co-PI: Nathan Crook, NC State CBE

Peptide-based adsorbents for affinity-based capture of next generation therapeutics

PI: Stefano Menegatti, NC State CBE
Co-PIs: Ruben Carbonell, NC State CBE; José Luis Martínez Ruiz, DTU Bioengineering

Nonwoven membranes for high-throughput chromatographic separations

PI: Ruben Carbonell, NC State CBE
Co-PIs: Stefano Menegatti, NC State CBE; Behnam Pourdeyhimi, NC State COT; John Woodley, DTU Chemical Engineering

Single-use technology for the flow-through polishing of protein therapeutics

PI: Stefano Menegatti, NC State CBE
Co-PIs: Ruben Carbonell, NC State CBE; John Woodley, DTU Chemical Engineering

**ISPR: Evaluation of in-situ product removal for therapeutic proteins**

PI: John Woodley, DTU Chemical Engineering
Co-PIs: Ruben Carbonell, NC State CBE; Stefano Menegatti, NC State CBE

Virtual pilot plant – Towards a digital process twin for efficient learning and experimental planning

PI: Krist Gernaey, DTU Chemical Engineering
Co-PIs: Gary Gilleskie, BTEC; Laurie Overton, BTEC

Other NC State faculty and staff contributing to these
projects include:

From DTU, also contributing to these research projects are:

Ulrich Krühne, Chemical Engineering
Seyed Soheil Mansouri, Chemical Engineering

Testing a novel chromatographic resin to see how well it can purify antibodies compared to traditional processes.

Key:

BME: Biomedical Engineering
CBE: Chemical and Biomolecular Engineering, College of Engineering
ECE: Electrical and Computer Engineering, College of Engineering
COT: College of Textiles